Journal: Ultrasonics Sonochemistry

Article Title: Ultrasound-assisted enzyme extraction of Citri Exocarpium Rubrum (Juhong) polysaccharides and its structural characteristics and evaluation of improving Parkinson’s disease through HDAC6/NLRP3 pathway

doi: 10.1016/j.ultsonch.2025.107636

Figure Lengend Snippet: UCERP1-1 attenuated the degeneration of dopaminergic neurons and neuron apoptosis. (A) The results of Nissl-positive neurons. n = 3, Scale bar = 100 μm, (magnification = 200×); (B) The quantification of Nissl cell in each group (n = 3); (C) The representative images of TH-positive neurons. n = 3, Scale bar = 100 μm, (magnification = 200×); (D) The quantification of TH+ cell in each group; (E) Western blotting analysis of α-Syn, and relative TH proteins in each group in F G NC MPTP L-DOPA UCERP1-1H UCERP1-1L A B D C α-Syn TH NC MPTP L-DOPA UCERP1-1H UCERP1-1L β-actin 15 KDa 57 KDa 42 KDa J E H I SNpc of MPTP-induced mice; (F) The quantification of relative α-Syn and TH proteins in each group (n = 3); (H) Cell viability was assessed using CCK8 assay; (I) Cell viability with MPP+ and UCERP1-1; (J) The level factor of LDH in the SH-SY5Y cells (n = 3); The data were presented as means ± SD, * P < 0.05, ** P < 0.01, vs. MPTP and MPP+ group. The statistical analysis was accomplished using one-way ANOVA and Tukey’s for multiple comparisons.

Article Snippet: MPP + was produced from Sigma (China), while L-DOPA (Med Chem Express LLC, USA) served as the positive control.

Techniques: Western Blot, CCK-8 Assay

Journal: Frontiers in behavioral neuroscience

Article Title: A mouse model of non-motor symptoms in Parkinson's disease: focus on pharmacological interventions targeting affective dysfunctions.

doi: 10.3389/fnbeh.2014.00290

Figure Lengend Snippet: FIGURE 5 | Effect of L-DOPA and pramipexole on depression- and anxiety-like behaviors in 6-OHDA-lesioned mice. Sham, and 6-OHDA-lesioned mice treated for 4 days with saline, L-DOPA (10 mg/kg), or pramipexole (0.6 mg/kg) were tested in (A) the forced swim test, or (B) the elevated plus maze test. Bar graphs show the immobility time (s) during the forced swim test (A; test duration, 10 min) and the time spent in the open arms (% of the test duration) during the elevated plus maze test (B; test duration 5 min). Data are expressed as mean ± s.e.m. (n = 4 −7). ∗∗p < 0.01, ∗∗∗p < 0.0001 vs. sham mice, ##p < 0.01,### p < 0.0001 vs. 6-OHDA-lesioned mice treated with saline, One-Way ANOVA, followed by Fisher post-hoc.

Article Snippet: The following drugs were used: benserazide hydrochloride, 3,4-dihydroxy-l-phenylalanine (L-DOPA), 6-hydroxydopamine hydrochloride (6-OHDA) and desipramine hydrochloride (Sigma-Aldrich, St. Louis, MO, USA), pramipexole dihydrochloride (Tocris, Bristol, UK) and reboxetine mesylate (Abcam Biochemicals, Cambridge, UK).

Techniques: Saline

Journal: Annals of Medicine

Article Title: Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression

doi: 10.1080/07853890.2024.2417179

Figure Lengend Snippet: Differential peripheral and central neurotransmitters in CSDS mice: (A) six differential neurotransmitters in the faeces of CSDS mice were identified; (B) three differential neurotransmitters in the Colon of CSDS mice were identified; (C) four differential neurotransmitters in the hippocampus of CSDS mice were identified; (D) eight differential neurotransmitters in the blood of CSDS mice were identified. Among these differential neurotransmitters, 3-OMDP, PEA, VMA, NE, L-DP, and DA belonged to the catecholaminergic pathway, and GABA, Orn, 5-GMP, Asp, Spn, Pun, GSH and Glu belonged to the GABAergic pathway. Each group had eight mice.

Article Snippet: 3-OMDP was purchased from MedChemExpress (HY-113468A, MedChemExpress, USA) and dissolved in a vehicle (sterile PBS) at a dosage of 3 mg/kg.

Techniques:

Journal: Annals of Medicine

Article Title: Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression

doi: 10.1080/07853890.2024.2417179

Figure Lengend Snippet: Correlations between differential gut microbiota and neurotransmitters: (A) close relationships were observed between differential genera and differential neurotransmitters. The number of neurotransmitters closely related with Limosilactobacillus was biggest, and 3-OMDP in blood was significantly correlated with five of six differential genera; (B) correlation analysis showed that the differential peripheral neurotransmitters could significantly affect central differential neurotransmitters, especially 5-GMP and 3-OMDP. Each group had eight mice.

Article Snippet: 3-OMDP was purchased from MedChemExpress (HY-113468A, MedChemExpress, USA) and dissolved in a vehicle (sterile PBS) at a dosage of 3 mg/kg.

Techniques:

Journal: Annals of Medicine

Article Title: Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression

doi: 10.1080/07853890.2024.2417179

Figure Lengend Snippet: Limosilactobacillus-3-OMDP-IL-1β/IL-6 as potential way in gut-brain axis. Here, the level of 3-OMDP was consistently decreased in faeces, Colon, blood and hippocampus of CSDS mice, and significant correlations were found between 3-OMDP in faeces and 3-OMDP in Colon, between 3-OMDP in Colon and 3-OMDP in blood, between 3-OMDP in blood and 3-OMDP in hippocampus. Meanwhile, we found that Limosilactobacillus was closely correlated with peripheral and Central 3-OMDP, and IL-1β was correlated with 3-OMDP. These results indicated that Limosilactobacillus might affect IL-6 and IL-1β levels in brain via peripheral and Central 3-OMDP, and then eventually leading to the onset of depression.

Article Snippet: 3-OMDP was purchased from MedChemExpress (HY-113468A, MedChemExpress, USA) and dissolved in a vehicle (sterile PBS) at a dosage of 3 mg/kg.

Techniques:

Journal: Annals of Medicine

Article Title: Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression

doi: 10.1080/07853890.2024.2417179

Figure Lengend Snippet: Effects of 3-OMDP on depressive-like behaviours in CSDS mice: ( A) sucrose preference (%) was significantly improved in CSDS mice receiving 3-OMDP than in CSDS mice receiving PBS; (B) immobility time was significantly decreased after 3-OMDP intervention in CSDS mice; (C) centre distance (%) was significantly increased after 3-OMDP intervention in CSDS mice. The similar sucrose preference (%), immobility time and centre distance (%) in CON+PBS group were similar to both CON + 3-OMDP group and CSDS + 3-OMDP group, but significantly different to CSDS+PBS group. These results indicated that 3-OMDP could significantly improve the depressive-like behaviours in CSDS mice. Each group had eight mice.

Article Snippet: 3-OMDP was purchased from MedChemExpress (HY-113468A, MedChemExpress, USA) and dissolved in a vehicle (sterile PBS) at a dosage of 3 mg/kg.

Techniques:

Journal: Annals of Medicine

Article Title: Limosilactobacillus-related 3-OMDP as a potential therapeutic target for depression

doi: 10.1080/07853890.2024.2417179

Figure Lengend Snippet: Effects of 3-OMDP on IL-1β and IL-6 in CSDS mice. After 3-OMDP intervention, the levels of IL-1β and IL-6 were significantly decreased in the hippocampus of CSDS + 3-OMDP group compared to CSDS+PBS group. CON+PBS group had similar levels of IL-1β and IL-6 compared to both CON + 3-OMDP group and CSDS + 3-OMDP group. These results showed that 3-OMDP could reverse the increased levels of IL-1β and IL-6 in the hippocampus of CSDS mice. Each group had eight mice.

Article Snippet: 3-OMDP was purchased from MedChemExpress (HY-113468A, MedChemExpress, USA) and dissolved in a vehicle (sterile PBS) at a dosage of 3 mg/kg.

Techniques: